WebOct 3, 2024 · The GsSir2/Ago complex presumably recognizes invading plasmid or phage DNA and activates the Sir2 subunit, which triggers endogenous NAD + depletion and cell … WebGsSir2/Ago-lacking E. coli MG1655 (shown in grey) or GsSir2/Ago-containing cells (shown in orange) were infected at t=0 at multiplicities of infection (MOI) of 0.05, 0.5 and 5. Each …
(PDF) Sir2-domain associated short prokaryotic …
WebThe GsSir2/Ago complex presumably recognizes invading plasmid or phage DNA and activates the Sir2 subunit, which triggers endogenous NAD+ depletion and cell death, … WebOct 3, 2024 · The GsSir2 protein contains a His 6-Tag at its N terminus. The mutants DSR2 (N133A) and DSR2 (H171A) were constructed using the Q5 Site-directed Mutagenesis … sanisoft technical services llc
The GsSir2/Ago system interferes with plasmid …
Web1 hour ago · The mechanism, guide/target preferences and function of short pAgos is an emerging topic in the Argonaute field, as evidenced by recent characterization of SPARTA and GsSir2/Ago antiviral systems ... Web1 hour ago · The mechanism, guide/target preferences and function of short pAgos is an emerging topic in the Argonaute field, as evidenced by recent characterization of … WebWe found that Ago C-terminal modification abolished protection from both phages (Fig. 1D). Thus, both the Sir2 and the Ago proteins are required for protection against phages by … short form of dhaka