Nettet28. jun. 2024 · Vmax and KM are the two parameters which define the kinetic behavior of an enzyme as a function of [S]. Vmax is a rate of reaction. It will have units of: or or etc. … NettetQuestion: Prepare Lineweaver Burke double reciprocal plots for each of the following enzymes. Refer to table 12−2 in your book to help with the inhibition questions. From …
Solved 3. Calculate KM and Vmax from the following data ... - Chegg
Nettet1. sep. 2024 · construct a Lineweaver-Burk plot, and assuming Michaelis-Menten kinetics, calculate the values of Vmax, Km, and k2 using the constructed plot. Q10.6b The data … NettetThe intercept of the line is 1/Vmax. So from the intercept you find Vmax. The slop of the line is Km/Vmax; by substituting the value you got for Vmax you can calculate the value of Km).... the young trustees movement
How to calculate Vmax and Km from a Lineweaver and …
NettetLineweaver-Burke: 1/ V o 1/[S] Inhibition Issue: changing the rate of enzyme activity in the cell (why?) ... V = Vmax [S]/([S]+Km) 1/V = (Km/Vmax)(1/[S]) + 1/ Vmax . For a fixed concentration of inhibitor and increasing substrate, expect the maximum to be the same, K m to increase V o Nettet1. sep. 2024 · Determine values for Vmax and Km. Solution Figure 13.12 shows the Lineweaver–Burk plot for this data and the resulting regression equation. Using the y -intercept, we calculate Vmax as Vmax = 1 / y −intercept = 1 / 1.708 mol = 0.585 mol … the young treasure hunter