Phosphopantetheine transferase

Author: tmwe

August undefined, 2024

Webfirst P-pant transferase to be cloned and characterized was the recently reported Escherichia co/i enzyme ACPS, ... each to catalyze the transfer of 4’-phosphopantetheine from.CoASH to apo-protein substrates. Results Database search for ACP synthase homologs BLAST searches (basic local alignment search tool) [12] ... WebMay 29, 2009 · The fungal type I fatty acid synthase (FAS) is a 2.6 MDa multienzyme complex, catalyzing all necessary steps for the synthesis of long acyl chains. To be catalytically competent, the FAS must be activated by a posttranslational modification of the central acyl carrier domain (ACP) by an intrinsic phosphopantetheine transferase (PPT).

UniProt

WebCoA- [4'-phosphopantetheine] + apo- acyl carrier protein adenosine 3',5'-bisphosphate + holo- acyl carrier protein This enzyme belongs to the family of transferases, specifically those … WebMay 30, 2024 · Phosphopantetheine adenylyltransferase (PPAT or CoaD) catalyses the penultimate step in the biosynthesis of CoA in prokaryotes. The enzyme catalyses the reversible transfer of an adenylyl group from ATP to 4′-phosphopantetheine (PhP) to yield 3′-dephospho-CoA (dpCoA) and pyrophosphate. reach registration fees

Cloning, expression, and characterization of a human 4 ...

WebPhosphopantetheine adenylyltransferase (PPAT, EC. 2.7.7.3) catalyzes an essential step in the reaction that transfers an adenylyl group from adenosine tri phosphate (ATP) to 4' … WebSep 17, 2013 · Phosphopantetheine transferases represent a class of enzymes found throughout all forms of life. From a structural point of view, they are subdivided into three groups, with transferases from group II being the most widespread. They are required for the posttranslational modification of carrier proteins involved in diverse metabolic pathways ... WebDec 14, 2011 · Function. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of an acyl-carrier-protein. The enzyme is able to transfer the cofactor to a broad range of enzymes with acyl- or peptidyl-carrier protein domains. Required for primary biological processes such as growth and asexual/sexual development, and activates target enzymes … reach registration number check

Characterization of a New Type of Phosphopantetheinyl …

The npgA/cfwA gene encodes a putative 4

WebPhosphopantetheine It is known as phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) or as the CoaD protein. From: Comprehensive Natural Products II, 2010 Peptide … WebNocardia phosphopantetheine transferase (PPTase) expressed in E. coli was isolated and characterized. When incubated with [3 H]acetyl-CoA and Nocardia PPTase, the labeled acetylphosphopantetheine moiety was incorporated into recombinant Car. reach registration number とはWebNov 13, 2013 · 4ihh: Chasing acyl carrier protein through a catalytic cycle of lipid A production. reach registration process

"WebPhosphopantetheinyl transferases (PPTases) are a superfamily of essential enzymes required for the synthetic processes of many compounds including fatty acid, … " - Phosphopantetheine transferase

Phosphopantetheine transferase

Cloning, expression, and characterization of a human 4 ...

Webtransferase (PPTase) interrogates the essential acyl carrier protein (ACP) domain to fulﬁll the initial activation step. The triggering factor of this study was the lack of structural information on PPTases at physiological pH, which could bias our comprehension of the mechanism of action of these important enzymes. WebThe structure of co-purified FAS shows discernible acyl carrier protein (ACP) domains and phosphopantetheine transferase (PPT) domains, which are responsible for substrate shuttling among ...

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WebMay 1, 2001 · A single candidate 4'-phosphopantetheine transferase, identified by BLAST searches of the human genome sequence data base, has been cloned, expressed, and characterized. The human enzyme, which is ... WebJun 18, 2003 · A single candidate 4'-phosphopantetheine transferase, identified by BLAST searches of the human genome sequence data base, has been cloned, expressed, and characterized. The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein.

WebNational Center for Biotechnology Information WebA single candidate 4'-phosphopantetheine transferase, identified by BLAST searches of the human genome sequence data base, has been cloned, expressed, and characterized.

WebJul 1, 2001 · Chapter 10 using phosphopantetheinyl transferases for enzyme posttranslational activation, site specific protein labeling and identification of natural product biosynthetic gene clusters from bacterial genomes. Murat Sunbul, Keya Zhang, Jun Yin Biology, Chemistry Methods in enzymology 2009 24 View 2 excerpts, cites background WebFeb 15, 2009 · The fungal type I fatty acid synthase (FAS) is a 2.6 MDa multienzyme complex, catalyzing all necessary steps for the synthesis of long acyl chains. To be catalytically competent, the FAS must be activated by a posttranslational modification of the central acyl carrier domain (ACP) by an intrinsic phosphopantetheine transferase (PPT) ...

Webphosphopantetheine transferase (PPTase), thus converting it to the active holoCP (3) (Fig. 1). Many organisms utilize more than one Ppant-dependent pathway. For instance, aside …

WebActivates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide antibiotics surfactin and plipastatin B1. Pfam Domain Function ACPS ( PF01648) reach registration searchPhosphopantetheine, also known as 4'-phosphopantetheine, is a prosthetic group of several acyl carrier proteins including the acyl carrier proteins (ACP) of fatty acid synthases, ACPs of polyketide synthases, the peptidyl carrier proteins (PCP), as well as aryl carrier proteins (ArCP) of nonribosomal peptide synthetases (NRPS). It is also present in formyltetrahydrofolate dehydrogenase. reach registrierte stoffeWebAlthough holo-acyl carrier protein synthase, AcpS, a phosphopantetheinyl transferase (PPTase), was characterized in the 1960s, it was not until the publication of the landmark … reach registration statusWebFeb 15, 2009 · The fungal type I fatty acid synthase (FAS) is a 2.6 MDa multienzyme complex, catalyzing all necessary steps for the synthesis of long acyl chains. To be catalytically competent, the FAS must be activated by a posttranslational modification of the central acyl carrier domain (ACP) by an intrinsic phosphopantetheine transferase (PPT). reach registryWebJun 6, 2024 · Unique CFP (cysteine-free protein; 120 aa) has been identified as an extraordinary virulence factor in Beauveria bassiana (Cordycipitaceae), a main source of wide-spectrum fungal insecticides. Its homologs exclusively exist in wide-spectrum insect pathogens of Hypocreales, suggesting their importance for a fungal insect-pathogenic … reach registration requirementsWebJul 1, 2002 · 1 Introduction. 4′-Phosphopantetheinyl transferases (PPTases) catalyze the conversion of 4′-phosphopantetheine (Ppant)-dependent carrier proteins from their inactive apo- into the active holo-form [1, 2].In this reaction, the Ppant moiety is derived from coenzyme A and transferred onto the side chain β-hydroxyl group of a conserved serine … reach registration polymersWebAn object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in … reach registry pad