WebJan 29, 2024 · Prior to beginning the study, a kill curve for the antibiotic and cell line should be established. This is performed as follows: Plate the cells at typical passaging ratio (e.g. 1:5, 1:10) into the plate or flask to be used in stable cell line creation. To the culture medium add concentrations of the antibiotic in a dilution series. WebStable cell selection and characterization. a Puromycin kill curve. A total of 1.5 μg/ml was the minimum concentration of puromycin in the cell culture medium required to kill non …

Has anyone done a HEK293 cells puromycin kill curve before?

Webpuromycin is 0.5 – 10 µg/ml. Different cell types and cell culture conditions may require different concentrations of selection antibiotic. Perform a kill curve to determine the … Web嘌呤霉素. 嘌呤霉素（Puromycin）是一种由白黑链霉菌产生的氨基苷类抗生素。. 其可以打乱核糖体上的肽转运，造成翻译过程中不成熟链终止，从而抑制蛋白质合成。. 在原核和真 … how fast can a shinkansen go

嘌呤霉素（Puromycin）使用说明书

Web- Plot these values on a graph to create a kill curve. The 'kill concentration' is the lowest concentration of puromycin which results in cell dealth of approximately 100% after 72 hours + 1µg/ml to account for difference in scale up. For example, the 'kill concentration in Fig. 2 is 3µg/ml. protocols.io Web初次做实验一定要先进行Puromycin梯度筛选预实验，建立杀死曲线（kill curve）。 1）以5~8×104 cells/孔的密度24孔板铺板，培养过夜。 2）准备筛选培养基：含不同浓度嘌呤霉 … WebCreate a Kill Curve This protocol is based on a standard suspension cell culture in a shaker flask. It can be scaled down appropriately to be performed in a 6-well, 12-well, or 24-well plate. 1. Seed at 1 × 106 ExpiCHO-S™ cells/mL five to ten 125-mL shake flasks in 30 mL of ExpiCHO™ Expression Medium. 2. Add selective pressure to each ... high court of justice in bankruptcy